Growth hormone stimulates osteoprotegerin expression and secretion in human osteoblast-like cells
نویسندگان
چکیده
منابع مشابه
Estrogen enhances growth hormone receptor expression and growth hormone action in rat osteosarcoma cells and human osteoblast-like cells.
Postmenopausal bone loss is primarily due to estrogen deficiency. Recent clinical observation demonstrate that GH increases bone mass in GH deficient patients. The present study investigates whether estrogen regulates GH action and GH receptor expression in osteoblasts. 17 beta-estradiol or GH added to the culture medium as single substances did not influence rat osteosarcoma cell proliferation...
متن کاملCGRP inhibits osteoprotegerin production in human osteoblast-like cells via cAMP/PKA-dependent pathway.
The osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL)/receptor activator of nuclear factor-kappaB (RANK) system was evaluated as a potential target of CGRP anabolic activity on bone. Primary cultures of human osteoblast-like cells (hOB) express calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1, and, because CGRP stimulates cAMP (one...
متن کاملIGF1Ec expression in MG-63 human osteoblast-like osteosarcoma cells.
AIM The insulin-like growth factor 1 (IGF1) gene gives rise to multiple transcripts, using an elaborate alternative splicing mechanism. The aim of this study was to shed light on the expression and role of the IGF1 system in human MG-63 osteoblast-like osteosarcoma cells. MATERIALS AND METHODS The expression of the IGF1Ea, IGF1Eb and IGF1Ec isoforms was characterized using reverse transcripti...
متن کاملEffects of Arachidonic Acid, Docosahexaenoic Acid, Prostaglandin E2 (PGE2), Oestrogen and Parathyroid Hormone on Osteoprotegerin (OPG) and RANKL Secretion by MG-63 and MC3T3-E1 Osteoblast-like Cells
Bone is continuously remodeled through resorption by osteoclasts and the subsequent synthesis of bone matrix by osteoblasts. (Refer to 2.4). The remodeling cycle is finely regulated by a variety of systemic and local factors e.g. oestrogen, parathyroid hormone (PTH), 1,25(OH)2D3, growth factors and cytokines. (Refer to 2.5). Cell-to-cell contact between osteoblasts (or bone marrow stroma cells)...
متن کاملHuman osteoblast-derived insulin-like growth factor (IGF) binding protein-5 stimulates osteoblast mitogenesis and potentiates IGF action.
Insulin-like growth factor (IGF)-binding proteins (IGFBPs) either inhibit or enhance IGF-stimulated cellular effects. While inhibition occurs by sequestration of IGF from cell-surface receptors, the exact mechanism of IGF-enhancement remains undefined. Human osteoblast-like bone cells in culture secrete several IGF-binding proteins, one of which we have previously identified as IGFBP-5. In this...
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ژورنال
عنوان ژورنال: Journal of Endocrinology
سال: 2007
ISSN: 0022-0795,1479-6805
DOI: 10.1677/joe.1.07073